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Registros recuperados: 58
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EXtreme ecosystem studies in the deep OCEan: Technological Developments. EXOCET/D ArchiMer
Sarradin, Pierre-marie; Sarrazin, Jozee; Cadiou, Jean-francois; Olu, Karine; Desbruyeres, Damien; Rigaud, Vincent; Drogou, Jean-francois; Lecornu, Fabrice; Rolin, Jean-francois; Vuillemin, Renaud; Cambon-bonavita, Marie-anne; Sauter, E; Von Juterzenka, K; Boetius, A; Santos, Rs; Colaco, Ana; Pascoal, A; Oliveira, Paulo; Shillito, Bruce; Zal, F; Schultz, A; Taylor, P; Lane, D; Loke, Rob; Du Buf, H; Waldmann, C; Cormack, A; Sanfilippo, L; Masson, M.
The general objective of EXOCET/D is to develop, implement and test specific instruments aimed at exploring, describing, quantifying and monitoring biodiversity in deep-sea fragmented habitats as well as at identifying links between community structure and environmental dynamics. Onboard experimental devices will complement the approach, enabling experiments on species physiology. The EXOCET/D working fields include: video and acoustic imagery, in situ analysis of physico-chemical factors, quantitative sampling of macroand micro-organisms, in vivo experiments, integration of multidisciplinary data, implementation on European deep-submersibles and a final phase of technical and scientific validation
Tipo: Text Palavras-chave: Deep sea; Ecosystem; Imagery; In situ analysis; Faunal sampling; Submersibles; Observatory.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2007/publication-3600.pdf
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ROV study of a giant pockmark on the Gabon continental margin ArchiMer
Ondreas, Helene; Olu, Karine; Fouquet, Yves; Charlou, Jean-luc; Gay, Aurelien; Dennielou, Bernard; Donval, Jean-pierre; Fifis, Alexis; Nadalig, Thierry; Cochonat, Pierre; Cauquil, E; Bourillet, Jean-francois; Le Moigne, Morgan; Sibuet, Myriam.
A giant, 800-m wide pockmark, called Regab, was discovered along the Equatorial African margin at 3160-m water depth and was explored by remote operated vehicle (ROV) as part of the Zaiango (1998-2000) and Biozaire (2001-2003) projects carried out conjointly by TOTAL and a number of French research institutes. A microbathymetric map obtained using the ROV sensors shows that the pockmark actually consists of a cluster of smaller pockmarks aligned N70 along a 15-m deep depression. Methane was recorded all over the pockmark, the highest values along the axis of the depression where massive carbonate crusts and dense seep communities were also found. Several faunal species belong to the Vesicomyidae and Mytilidae bivalve families, as well as to Siboglinidae...
Tipo: Text Palavras-chave: Gabon; Microbathymetric map; ROV; Exploration; Pockmark; Equatorial African margin.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1239.pdf
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Comments on the paper of Gay et al. (2006) Seafloor facies related to upward methane flux within a Giant Pockmark of the Lower Congo Basin. Marine Geology 226 : 81-95 ArchiMer
Olu, Karine.
The paper by Gay et al. describes a giant pockmark on the basis of geological and biological data collected during ROV dives. The description of seep communities based on a transect across the pockmark includes some mistakes. Part of the description of the fauna is attributed to Olu-Le Roy et al. (2003) and includes data that have not been presented in this oral communication. An "evolutive model" is proposed, based on these data and on an incorrect interpretation of symbiotic species requirements (classification of methane vs sulfide dependent species). In this note, I would like to point out the mistakes of this paper and express why their model cannot be used to describe the reality of the seabed.
Tipo: Text Palavras-chave: BIOZAIRE; ZAIANGO; Palaeochannel; Hydrates; Chemosynthesis; Seafloor facies; Fluid migration; Pockmark.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2276.pdf
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Evidence for chemoautotrophic symbiosis in a Mediterranean cold seep clam (Bivalvia : Lucinidae): comparative sequence analysis of bacterial 16S rRNA, APS reductase and RubisCO genes ArchiMer
Duperron, Sebastien; Fiala Medioni, Aline; Caprais, Jean-claude; Olu, Karine; Sibuet, Myriam.
Symbioses between lucinid clams (Bivalvia: Lucinidae) and autotrophic sulphide-oxidizing bacteria have mainly been studied in shallow coastal species, and information regarding deep-sea species is scarce. Here we study the symbiosis of a clam, resembling Lucinoma kazani, which was recently collected in sediment cores from new cold-seep sites in the vicinity of the Nile deep-sea fan, eastern Mediterranean, at depths ranging from 507 to 1691 m. A dominant bacterial phylotype, related to the sulphide-oxidizing symbiont of Lucinoma aequizonata, was identified in gill tissue by comparative 16S rRNA gene sequence analysis. A second phylotype, related to spirochete sequences, was identified twice in a library of 94 clones. Comparative analyses of gene sequences...
Tipo: Text Palavras-chave: Eastern Mediterranean; Cold seeps; Lucinoma; Lucinidae; Sulphide oxidizing bacteria; Symbiosis.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2176.pdf
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Amphi-Atlantic cold-seep Bathymodiolus species complexes across the equatorial belt ArchiMer
Olu, Karine; Von Cosel, R; Hourdez, S; Carney, S; Jollivet, D.
Deep-sea bivalves of the subfamily Bathymodiolinae (family Mytilidae) are very widespread and form dense beds in reduced environments such as hydrothermal vents and cold seeps. Bathymodiolus mussels recently discovered on African cold seeps strangely resemble Gulf of Mexico and Barbados seep species. This raises intriguing questions regarding their taxonomic relationships and their dispersal capabilities across the Atlantic equatorial belt. The morphological study of the shell and soft parts of mussels from either sites of the Atlantic shows that they form two distinct groups: the Bathymodiolus boomerang group (also including Bathymodiolus heckerae and a species from Africa), and the Bathymodiolus childressi group (also including Bathymodiolus mauritanicus...
Tipo: Text Palavras-chave: RDNA ITS2; Mitochondrial cytochrome oxydase; Amphi Atlantic species; Cold seeps; Bathymodiolus.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4064.pdf
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Cold-seep assemblages on a giant pockmark off West Africa: spatial patterns and environmental control ArchiMer
Olu, Karine; Caprais, Jean-claude; Fifis, Alexis; Fabri, Marie-claire; Galeron, Joelle; Budzinsky, H; Le Menach, K; Khripounoff, Alexis; Ondreas, Helene; Sibuet, Myriam.
A giant pockmark colonised by dense cold-seep assemblages near 3160 m depth along the Congo-Angola margin has been surveyed by the ROV Victor 6000. The quantitative distribution of chemosynthetic communities was mapped along the dive tracks from a video study using GIS and image mosaicking. Several types of faunal assemblages, either dominated by bivalves of the families Mytilidae (Bathmodiolus sp.) Vesicom idae (Calyptogena sp., 'Vesicomya' aff. chuni), or by Siboglinidae polychaetes (Escarpia southwardae) were mapped over the 800-m diameter pockmark area and sampled for fauna, water and sediment. The isotopic analyses (delta C-13) of tissues from symbiont-bearing species were within the range typical of nutrition via symbiosis using methane for mussels...
Tipo: Text Palavras-chave: Symbiont bearing species; Spatial distribution; Methane; Megafauna; Cold seeps.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2681.pdf
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Seafloor geological studies above active gas chimneys off Egypt (Central nile deep sea fan) ArchiMer
Dupre, Stephanie; Woodside, J; Foucher, Jean-paul; De Lange, G; Mascle, J; Boetius, A; Mastalerz, V; Stadnitskaia, A; Ondreas, Helene; Huguen, C; Harmegnies, Francois; Gontharet, Swanne; Loncke, L; Deville, E; Niemann, H; Omoregie, E; Olu, Karine; Fiala Medioni, A; Dahlmann, A; Caprais, Jean-claude; Prinzhofer, A; Sibuet, Myriam; Pierre, C; Damste, J.
Four mud volcanoes of several kilometres diameter named Amon, Osiris, Isis, and North Alex and located above gas chimneys on the Central Nile Deep Sea Fan, were investigated for the first time with the submersible Nautile. One of the objectives was to characterize the seafloor morphology and the seepage activity across the mud volcanoes. The seepage activity was dominated by emissions of methane and heavier hydrocarbons associated with a major thermal contribution. The most active parts of the mud volcanoes were highly gas-saturated (methane concentrations in the water and in the sediments, respectively, of several hundreds of nmol/L and several mmol/L of wet sediment) and associated with significantly high thermal gradients (at 10 m below the seafloor,...
Tipo: Text Palavras-chave: Seafloor morphology; Methane; Authigenic carbonate precipitation; Gas chimneys; Mud breccia; Mud volcanoes; Fluid seepage; Nile fan.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4455.pdf
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The Magellan mound province in the Porcupine Basin ArchiMer
Huvenne, Veerle A. I.; Bailey, W. R.; Shannon, P. M.; Naeth, J; Di Primio, R.; Henriet, J. P.; Horsfield, B.; De Haas, H.; Wheeler, A; Olu, Karine.
The Magellan mound province is one of the three known provinces of carbonate mounds or cold-water coral banks in the Porcupine Seabight, west of Ireland. It has been studied in detail using a large and varied data set: 2D and 3D seismic data, sidescan sonar imagery and video data collected during ROV deployment have been used to describe the mounds in terms of origin, growth processes and burial. The aim of this paper is to present the Magellan mounds and their setting in an integrated, holistic way. More than 1,000 densely spaced and mainly buried mounds have been identified in the area. They all seem to be rooted on one seismic reflection, suggesting a sudden mound start-up. Their size and spatial distribution characteristics are presented, together with...
Tipo: Text Palavras-chave: Numerical basin modelling; Fault analysis; Mound morphology; Spatial distribution; Porcupine Basin; Cold water corals; Carbonate mounds.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2312.pdf
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Morphology and environment of cold-water coral carbonate mounds on the NW European margin ArchiMer
Wheeler, Andrew J.; Beyer, A; Freiwald, A; De Haas, H; Huvenne, V. A. I.; Kozachenko, M; Olu, Karine; Opderbecke, Jan.
Cold-water coral carbonate mounds, owing their presence mainly to the framework building coral Lophelia pertusa and the activity of associated organisms, are common along the European margin with their spatial distribution allowing them to be divided into a number of mound provinces. Variation in mound attributes are explored via a series of case studies on mound provinces that have been the most intensely investigated: Belgica, Hovland, Pelagia, Logachev and Norwegian Mounds. Morphological variation between mound provinces is discussed under the premise that mound morphology is an expression of the environmental conditions under which mounds are initiated and grow. Cold-water coral carbonate mounds can be divided into those exhibiting "inherited"...
Tipo: Text Palavras-chave: Seabed mapping; Environmental setting; Morphology; Cold water coral; Carbonate mound.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2411.pdf
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Therese Mound: a case study of coral bank development in the Belgica Mound Province, Porcupine Seabight ArchiMer
De Mol, Ben; Kozachenko, M; Wheeler, Andy; Alvares, Hugo; Henriet, Jean-pierre; Olu, Karine.
High-resolution seismic profiles, swath bathymetry, side-scan sonar data and video imageries are analysed in this detailed study of five carbonate mounds from the Belgica mound province with special emphasis on the well-surveyed Therese Mound. The selected mounds are located in the deepest part of the Belgica mound province at water depths of 950 m. Seismic data illustrate that the underlying geology is characterised by drift sedimentation in a general northerly flowing current regime. Sigmoidal sediment bodies create local slope breaks on the most recent local erosional surface, which act as the mound base. No preferential mound substratum is observed, neither is there any indication for deep geological controls on coral bank development. Seismic evidence...
Tipo: Text Palavras-chave: Carbonate mounds; Cold water corals; Lophelia pertusa; Porcupine Seabight; Coral banks; Belgica mound province; Therese Mound.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2307.pdf
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The distribution of scleractinian corals in the Bay of Biscay, NE Atlantic ArchiMer
Reveillaud, J; Freiwald, A; Van Rooij, D; Le Guilloux, Erwan; Altuna, A; Foubert, A; Vanreusel, A; Olu, Karine; Henriet, J.
Studies on the distribution of scleractinian corals in the Bay of Biscay were relatively numerous during the nineteenth and the first half of the twentieth century. Yet, recent reports are scattered, sometimes unpublished, and therefore knowledge about the current coral occurrence in the area is limited. This study aims at compiling the available historical and more recently collected information on the occurrence of scleractinian corals in the Bay of Biscay. Data from two recent cruises are included and compared with previous explored coral sites from as early as 1830 up to 1995. A database of 347 records including 34 described species of scleractinians highlights that the slope of the Bay of Biscay is an important habitat for scleractinians. This could...
Tipo: Text Palavras-chave: Biodiversity; Bay of Biscay; Slope; Scleractinia; Cold water corals.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4503.pdf
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First observations of deep-sea coral reefs along the Angola margin ArchiMer
Le Guilloux, E.; Olu, Karine; Bourillet, Jean-francois; Savoye, Bruno; Iglesias, S. P.; Sibuet, Myriam.
The West African continental slope is an important theatre for geological survey prospecting and drilling for hydrocarbons but little is known about local deep-sea biological communities at these depths. While shallow-water reefs are common and well-known features in the tropics, only few records of deep-water corals exist at low latitudes, and most of them have been reported by historical oceanographic cruises undertaking circum-navigations of the world. This study, based on a multi-disciplinary approach, presents a description of newly discovered deep-water coral reef communities along the Angola margin. Data from ROV, multibeam bathymetry, side-scan sonar and seismics from a deep-towed acoustic system (SAR) were used to describe the morphology of the...
Tipo: Text Palavras-chave: Lucinids; Habitat mapping; ROV; Lophelia pertusa; Deep sea corals; Angola margin.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7403.pdf
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Trophic relationships in a deep Mediterranean cold-water coral bank (Santa Maria di Leuca, Ionian Sea) ArchiMer
Carlier, A.; Le Guilloux, E.; Olu, Karine; Sarrazin, Jozee; Mastrototaro, F.; Taviani, M.; Clavier, J..
Cold-water corals (CWC) are frequently reported from deep sites with locally accelerated currents that enhance seabed food particle supply. Moreover, zooplankton likely account for ecologically important prey items, but their contribution to CWC diet remains unquantified. We investigated the benthic food web structure of the recently discovered Santa Maria di Leuca (SML) CWC province (300 to 1100 m depth) located in the oligotrophic northern Ionian Sea. We analyzed stable isotopes (delta C-13 and delta N-15) of the main consumers (including ubiquitous CWC species) exhibiting different feeding strategies, zooplankton, suspended particulate organic matter (POM) and sedimented organic matter (SOM). Zooplankton and POM were collected 3 m above the coral...
Tipo: Text Palavras-chave: Particulate organic matter; Zooplankton; Stable isotopes; Food web; Mediterranean Sea; Benthic community; Cold water corals.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7450.pdf
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Large Vesicomyidae (Mollusca: Bivalvia) from cold seeps in the Gulf of Guinea off the coasts of Gabon, Congo and northern Angola ArchiMer
Von Cosel, Rudo; Olu, Karine.
Two new genera and three new species of large Vesicomyidae are described from cold-seep sites on pockmarks and other sulfide-rich environments in the Gulf of Guinea (tropical east Atlantic) off Gabon, Congo (Brazzaville) and northern Angola, from 500 to 4000 m depth: "Catyptogena" (s.l.) regab n. sp., Wareniconcha (n.g.) guineensis (Thiele and Jaeckel 1931), Elenaconcha guiness n.g. n. sp., and Isorropodon atalantae n. sp. For two other species already taken by the R/V Valdivia in 1898, Calyptogena valdiviae (Thiele and Jaeckel 1931) and Isorropodon striatum (Thiele and Jaeckel 1931) new localities were discovered, and the species are rediscussed. E. guiness n.g. n.sp. is also recorded from off Banc d'Arguin, Mauritania, collected by commercial fishing...
Tipo: Text Palavras-chave: New Taxa; Systematics; Eastern Atlantic; Gulf of Guinea; Cold seeps; Vesicomyidae.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7443.pdf
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Influence of seep emission on the non-symbiont-bearing fauna and vagrant species at an active giant pockmark in the Gulf of Guinea (Congo-Angola margin) ArchiMer
Olu, Karine; Caprais, Jean-claude; Galeron, Joelle; Causse, R.; Von Cosel, R.; Budzinski, H.; Le Menach, K.; Le Roux, C.; Levache, D.; Khripounoff, Alexis; Sibuet, Myriam.
Detailed surveying with an ROV found that a dense and diverse cold-seep community colonises a giant pockmark located at 3200 m depth, 8 km north from the deep Congo channel. Several types of assemblages, either dominated by Mytilidae and Vesicomyidae bivalves or Siboglinidae polychaetes, are distributed on the 800-m diameter active area. The site is characterised by a most active central zone in a depression with abundant carbonate concretions and high methane fluxes where high-density clusters of mussels and siboglinids dominate. In contrast, the peripheral zones display large fields of dead and live vesicomyids on soft sediment, with a lower mean density and lower methane concentration in seawater. The associated megafauna includes Alvinocarididae...
Tipo: Text Palavras-chave: Congo Angola margin; Atlantic Equatorial African margin; Vagrant species; Isotopic signature; Megafauna; Cold seep.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7478.pdf
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Biodiversity of Cold Seep Ecosystems Along the European Margins ArchiMer
Vanreusel, Ann; Andersen, Ann C.; Boetius, Antje; Connelly, Douglas; Cunha, Marina R.; Decker, Carole; Hilario, Ana; Kormas, Konstantinos Ar.; Maignien, Lois; Olu, Karine; Pachiadaki, Maria; Ritt, Benedicte; Rodrigues, Clara; Sarrazin, Jozee; Tyler, Paul; Van Gaever, Saskia; Vanneste, Heleen.
During the European Commission's Framework Six Programme, HERMES, we investigated three main areas along the European margin, each characterized by the presence of seep-related structures exhibiting different intensity of activity and biological diversity. These areas are: (1) the Nordic margin with the Hakon Mosby mud volcano and many pockmarks, (2) the Gulf of Cadiz, and (3) the eastern Mediterranean with its hundreds of mud volcanoes and brine pool structures. One of the main goals of the HERMES project was to unravel the biodiversity associated with these seep-associated ecosystems, and to understand their driving forces and functions, using an integrated approach. Several multidisciplinary research cruises to these three areas provided evidence of...
Tipo: Text Palavras-chave: Mosby mud volcano; Gulf of mexico; Cadiz ne atlantic; Deep sea fan; Community structure; Microbial communities; Fluid flow; Mediterranean sea; Carbonate crusts; Norwegian sea.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6375.pdf
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Metazoan meiofaunal communities at cold seeps along the Norwegian margin: Influence of habitat heterogeneity and evidence for connection with shallow-water habitats ArchiMer
Van Gaever, Saskia; Olu, Karine; Derycke, Sofie; Vanreusel, Ann.
Cold-seep environments and their associated symbiont-bearing megafaunal communities create islands of primary production for macro- and meiofauna in the otherwise monotonous and nutrient-poor deep-sea environment. To examine the spatial variation and distribution patterns of metazoan meiobenthos in different seepage-related habitats, samples were collected in two regions off Norway: several pockmarks associated with the Storegga Slide including the Nyegga pockmark area (730 m: 64 degrees N), and the active, methane-venting Hakon Mosby Mud Volcano (HMMV) west of the Barents Sea (1280 m: 72 degrees N). Based on sediment geochemistry and associated epifauna, three different habitat types were distinguished across the two regions: (1) reduced sediment with...
Tipo: Text Palavras-chave: Terschellingia longicaudata; Halomonhystera disjuncta; Cryptic species; Nematodes; Meiobenthos; Cold seeps.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7484.pdf
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Spatial heterogeneity of macrofaunal communities in and near a giant pockmark area in the deep Gulf of Guinea ArchiMer
Menot, Lenaick; Galeron, Joelle; Olu, Karine; Caprais, Jean-claude; Crassous, Philippe; Khripounoff, Alexis; Sibuet, Myriam.
The structure and familial-level composition of macrofaunal communities were examined within and at distance from the giant pockmark Regab in the Southern Gulf of Guinea, at 3200 m depth. The two main questions addressed in this study were (i) does habitat partitioning by large symbiont-bearing taxa. influence macrofaunal assemblages and their environment? and (ii) to what extent does the chemosynthesis-based ecosystem influence the structure and the composition of nearby macrobenthic communities? Along two radials, at sites from 250 to 1000 m away from the active centre of the cold seep, the abundance and composition of the macrofauna were typical of a deep-sea community at that depth. Except for a few cores sampled in or near dead vesicomyid fields at...
Tipo: Text Palavras-chave: Cold seep; Diversity; Gulf of Guinea; Habitat heterogeneity; Macrofauna; Open slope; Siboglinid tubeworms; Vesicomyid clams.
Ano: 2010 URL: https://archimer.ifremer.fr/doc/00002/11282/8099.pdf
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Biogeography and Potential Exchanges Among the Atlantic Equatorial Belt Cold-Seep Faunas ArchiMer
Olu, Karine; Cordes, Erik E.; Fisher, Charles R.; Brooks, James M.; Sibuet, Myriam; Desbruyeres, Daniel.
Like hydrothermal vents along oceanic ridges, cold seeps are patchy and isolated ecosystems along continental margins, extending from bathyal to abyssal depths. The Atlantic Equatorial Belt (AEB), from the Gulf of Mexico to the Gulf of Guinea, was one focus of the Census of Marine Life ChEss (Chemosynthetic Ecosystems) program to study biogeography of seep and vent fauna. We present a review and analysis of collections from five seep regions along the AEB: the Gulf of Mexico where extensive faunal sampling has been conducted from 400 to 3300m, the Barbados accretionary prism, the Blake ridge diapir, and in the Eastern Atlantic from the Congo and Gabon margins and the recently explored Nigeria margin. Of the 72 taxa identified at the species level, a total...
Tipo: Text Palavras-chave: Gulf of mexico; Barbados accretionary prism; Mussel bathymodiolus childressi; Hydrothermal vent communities; Deep sea vent; Spatial distribution; Family veiscomyidae; Dispersal barriers; Hydrocarbon seeps; Species diversity.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12207/8975.pdf
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Heterogeneous energetic pathways and carbon sources on deep eastern Mediterranean cold seep communities ArchiMer
Carlier, Antoine; Ritt, Benedicte; Rodrigues, Clara F.; Sarrazin, Jozee; Olu, Karine; Grall, Jacques; Clavier, Jacques.
Cold seep communities in the Mediterranean Sea have only been discovered two decades ago, and their trophic ecology has been the subject of very few studies. We investigated the benthic food web of two deep chemosynthesis-based ecosystems on the Napoli and Amsterdam mud volcanoes (MVs) in the eastern Mediterranean Sea (similar to 2,000 m depth). Seeping methane has been detected at the surface of both MVs during pioneering cruises and has been hypothesised to be assimilated by benthic fauna as observed in other oceans' margins. Given the extreme oligotrophic character of the eastern Mediterranean Sea, we a priori expected that chemosynthetic food sources, especially methane-derived carbon (MDC), played a major trophic role in these deep seep communities...
Tipo: Text Palavras-chave: Gulf of Mexico; Anaerobic methane oxidation; Stable isotope analysis; Sea hydrothermal vents; Food web structure; Kazan mud volcano; Florida escarpment; Trophic relationships; Benthic communauties; Lamellibrachia SP.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00016/12684/9633.pdf
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